An Optimised Cetyltrimethylammonium Bromide (CTAB)-Based Protocol for Extracting RNA from Young and Old Cassava Leaves
نویسندگان
چکیده
منابع مشابه
Silica nanoparticles separation from water: aggregation by cetyltrimethylammonium bromide (CTAB).
Nanoparticles will inevitably be found in industrial and domestic wastes in the near future and as a consequence soon in water resources. Due to their ultra-small size, nanoparticles may not only have new hazards for environment and human health, but also cause low separation efficiency by classical water treatments processes. Thus, it would be an important challenge to develop a specific treat...
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Isolation of high-quality RNA free of contaminants, such as polyphenols, proteins, plant secondary metabolites, and genomic DNA from plant tissues, is usually a challenging but crucial step for molecular analysis. We developed a novel protocol based on the cetyltrimethylammonium bromide method to isolate high-quality RNA from blackberry plant tissues, especially fruits. Most DNA was remove...
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The present research attempt consists in the development of mesoporous hydroxyapatite using inexpensive ingredients using cetyltrimethylammonium bromide (CTAB) as template through a chemical-based hydrothermal method. Wide angle X-ray diffraction (XRD) reveals the formation of pure single-phased hydroxyapatite particles. Fourier transform infrared absorption spectroscopy (FTIR) and electron dif...
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We have characterized the phase behavior of mixtures of the cationic surfactant cetyltrimethylammonium bromide (CTAB) and the organic salt 3-sodium-2-hydroxy naphthoate (SHN) over a wide range of surfactant concentrations using polarizing optical microscopy and X-ray diffraction. A variety of liquid crystalline phases, such as hexagonal, lamellar with and without curvature defects, and nematic,...
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ZnO nanostructures were synthesized by hydrothermal method using different molar ratios of cetyltrimethylammonium bromide (CTAB) and Sodium dodecyl sulfate (SDS) as structure directing agents. The effect of surfactants on the morphology of the ZnO crystals was investigated by field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM) techniques. The results i...
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ژورنال
عنوان ژورنال: Journal of Advances in Biology & Biotechnology
سال: 2018
ISSN: 2394-1081
DOI: 10.9734/jabb/2018/45370